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G3BP1 Monoclonal antibody
G3BP1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig and More (1)
Applications
IF/ICC, IHC, WB, ELISA and More (4)
Conjugate
Unconjugated
CloneNo.
1E4A2
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, RAW 264.7 cells, pig brain tissue, HeLa cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human testis tissue, human colon tissue, human lymphoma tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | sodium arsenite treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66486-1-Ig targets G3BP1 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | CoIP, FC, IF, IP, RIP, WB |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Immunogen | G3BP1 fusion protein Ag3728 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Synonyms | G3BP, G3BP 1, G3BP1, HDH VIII |
| Calculated Molecular Weight | 466 aa, 52 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC006997 |
| Gene Symbol | G3BP1 |
| Gene ID (NCBI) | 10146 |
| RRID | AB_2819031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13283 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for G3BP1 antibody 66486-1-Ig | Download protocol |
| IHC protocol for G3BP1 antibody 66486-1-Ig | Download protocol |
| IF protocol for G3BP1 antibody 66486-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome. | ||
Sci Transl Med Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Autophagy Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules | ||
Nat Chem Biol K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle. |
