FGFR1 Monoclonal antibody

FGFR1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

10F12E7

Cat No : 60325-1-Ig

Print datasheet

Synonyms

bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr



经过测试的应用

Positive WB detected inMCF-7 cells, HT-1080 cells, HUVEC cells, U2OS cells, Y79 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60325-1-Ig targets FGFR1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FGFR1 fusion protein Ag3646 种属同源性预测
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Full Name fibroblast growth factor receptor 1
Synonyms bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr
Calculated Molecular Weight 820 aa, 91 kDa
Observed Molecular Weight 91 kDa
GenBank Accession NumberBC018128
Gene Symbol FGFR1
Gene ID (NCBI) 2260
RRIDAB_2881435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.

实验方案

Product Specific Protocols
WB protocol for FGFR1 antibody 60325-1-IgDownload protocol
IHC protocol for FGFR1 antibody 60325-1-IgDownload protocol
FC protocol for FGFR1 antibody 60325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
humanWB

Theranostics

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Authors - Meng Wang
humanWB,IHC

J Transl Med

A novel intronic circular RNA circFGFR1int2 up-regulates FGFR1 by recruiting transcriptional activators P65/FUS and suppressing miR-4687-5p to promote prostate cancer progression

Authors - Ruyue Wang
humanWB

Oxid Med Cell Longev

DJ-1/FGFR-1 Signaling Pathway Contributes to Sorafenib Resistance in Hepatocellular Carcinoma.

Authors - Xin Chen
humanWB

Int J Mol Sci

The FGFR Family Inhibitor AZD4547 Exerts an Antitumor Effect in Ovarian Cancer Cells.

Authors - Yu Ran Na
WB

J Cell Physiol

Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD.

Authors - Lele Li