FBXO32 Monoclonal antibody

FBXO32 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rat, mouse and More (4)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat No : 67172-1-Ig

Print datasheet

Synonyms

Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein



经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human heart tissue, pig heart tissue, pig skeletal muscle tissue, Rabbit skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy FBXO32 Ready-To-Use IHC Kit. FBXO32 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67172-1-Ig targets FBXO32 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Rat, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity Human, Pig, Rat, mouse
Cited Reactivityhuman, mouse, rat, pig, canine
Immunogen FBXO32 fusion protein Ag25247 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name F-box protein 32
Synonyms Atrogin 1, F box only protein 32, F box protein 32, Fbx32, FBXO32, FLJ32424, MAFbx, Muscle atrophy F box protein
Calculated Molecular Weight 355 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC024030
Gene Symbol FBXO32
Gene ID (NCBI) 114907
RRIDAB_2882468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969P5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

实验方案

Product Specific Protocols
WB protocol for FBXO32 antibody 67172-1-IgDownload protocol
IHC protocol for FBXO32 antibody 67172-1-IgDownload protocol
IF protocol for FBXO32 antibody 67172-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Biol Toxicol

Statins induce skeletal muscle atrophy via GGPP depletion-dependent myostatin overexpression in skeletal muscle and brown adipose tissue.

Authors - Lai Wang
pigWB

J Anim Sci Biotechnol

Protective effects of taurine against muscle damage induced by diquat in 35 days weaned piglets.

Authors - Chaoyue Wen
canineWB,IHC

Life Sci

N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway

Authors - Qingyu Ding
mouseWB

J Ethnopharmacol

Saponin extract from Achyranthes bidentata Blume alleviates disuse-induced muscle atrophy through PI3K/Akt signaling pathway

Authors - Yi Shi
mouseWB

Front Cell Dev Biol

Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.

Authors - Xiangyu Mao
mouseWB

Exp Cell Res

Resistance exercise alleviates dexamethasone-induced muscle atrophy via Sestrin2/MSTN pathway in C57BL/6J mice

Authors - Yang Yang