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ETHE1 Polyclonal antibody

ETHE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27786-1-AP

Print datasheet

Synonyms

ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12

验证数据展示

  • WB analysis of BGC-823 using 27786-1-AP

    WB analysis of BGC-823 using 27786-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27786-1-AP

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ETHE1 antibody included
  • IHC staining of human liver using 27786-1-AP

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ETHE1 antibody included

经过测试的应用

Positive WB detected inBGC-823 cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ETHE1 Ready-To-Use IHC Kit. ETHE1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27786-1-AP targets ETHE1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen ETHE1 fusion protein Ag26597 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ethylmalonic encephalopathy 1
Synonyms ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC008250
Gene Symbol ETHE1
Gene ID (NCBI) 23474
RRIDAB_2880971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95571
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ETHE1, a gene encoding a b-lactamase-like, iron-coordinating metalloprotein, is mutated in ethylmalonic encephalopathy.In humans, ETHE1 gene is proved to encode the SDO in mitochondria and its mutations lead to a fatal autosomal recessive mitochondrial disease: ethylmalonic encephalopathy.

实验方案

Product Specific Protocols
WB protocol for ETHE1 antibody 27786-1-APDownload protocol
IHC protocol for ETHE1 antibody 27786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols