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  • KD/KO Validated

ERO1LB Polyclonal antibody

ERO1LB Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11261-2-AP

Print datasheet

Synonyms

ERO1 L beta, ERO1 like beta (S. cerevisiae), ERO1 like protein beta, ERO1B, ERO1LB, Oxidoreductin 1 L beta



经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue
Positive IHC detected inhuman pancreas cancer tissue, human oesophagus tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11261-2-AP targets ERO1LB in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen ERO1LB fusion protein Ag1776 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ERO1-like beta (S. cerevisiae)
Synonyms ERO1 L beta, ERO1 like beta (S. cerevisiae), ERO1 like protein beta, ERO1B, ERO1LB, Oxidoreductin 1 L beta
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 40~50 kDa
GenBank Accession NumberBC032823
Gene Symbol ERO1LB
Gene ID (NCBI) 56605
RRIDAB_2101503
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.

实验方案

Product Specific Protocols
WB protocol for ERO1LB antibody 11261-2-APDownload protocol
IHC protocol for ERO1LB antibody 11261-2-APDownload protocol
IF protocol for ERO1LB antibody 11261-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Curcumin Facilitates a Transitory Cellular Stress Response in Trembler-J Mice.

Authors - Okamoto Yuji Y
mouseWB

Endocrinology

PMID: 21540283

Authors - Khoo Cynthia C
  • KD Validated
humanWB

J Biol Chem

Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells.

Authors - Rajpal Gautam G
humanWB

Sci Rep

Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells.

Authors - Norio Takei
  • KO Validated
mouseWB

J Toxicol

Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease.

Authors - Galligan James J JJ
humanWB

ACS Bio Med Chem Au

Identification of Natural Product Sulfuretin Derivatives as Inhibitors for the Endoplasmic Reticulum Redox Protein ERO1α

Authors - Brennan D Johnson
  • KO Validated