CoraLite®594-conjugated ERK1/2 Monoclonal antibody
ERK1/2 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4G8A11
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66192-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66192 targets ERK1/2 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | human, mouse |
Immunogen | ERK1/2 fusion protein Ag20350 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase 3 |
Synonyms | ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3 |
Calculated Molecular Weight | 43 kDa |
GenBank Accession Number | BC013992 |
Gene Symbol | MAPK3 |
Gene ID (NCBI) | 5595 |
RRID | AB_2883530 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P27361 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 ERK1/2 antibody CL594-66192 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |