EPO Monoclonal antibody

EPO Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (2)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11F3

Cat No : 66975-1-Ig

Print datasheet

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2



经过测试的应用

Positive WB detected inrecombinant human EPO (cat.NO. HZ-1168)
Positive IHC detected inhuman liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

66975-1-Ig targets EPO in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited Applications WB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen EPO fusion protein Ag23054 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name erythropoietin
Synonyms EP, EPO, Epoetin, erythropoietin, MVCD2
Calculated Molecular Weight 193 aa, 21 kDa
GenBank Accession NumberBC093628
Gene Symbol EPO
Gene ID (NCBI) 2056
RRIDAB_2882295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

实验方案

Product Specific Protocols
WB protocol for EPO antibody 66975-1-IgDownload protocol
IHC protocol for EPO antibody 66975-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2.

Authors - Meng Wang
ratWB

Front Cardiovasc Med

Molecular and Cellular Response of the Myocardium (H9C2 Cells) Towards Hypoxia and HIF-1α Inhibition.

Authors - Hari Prasad Osuru
mouseWB

Am J Physiol Renal Physiol

The hypoxia-inducible factor-α prolyl hydroxylase inhibitor FG4592 ameliorates renal fibrosis by inducing the H3K9 demethylase JMJD1A

Authors - Takeshi Ike
mouseWB

ChemMedChem

Perfluorocarbon Nanoparticles Incorporating GB: Artificial O2 Carrier with Antioxidant Activity and Antithrombotic Effect

Authors - Wei Meng