Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28117-1-AP

Print datasheet

Synonyms

CD105, END, Endoglin, ENG, FLJ41744, HHT1, ORW, ORW1



经过测试的应用

Positive WB detected inHUVEC cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

28117-1-AP targets Endoglin/CD105 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited Applications IHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Endoglin/CD105 fusion protein Ag27822 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endoglin
Synonyms CD105, END, Endoglin, ENG, FLJ41744, HHT1, ORW, ORW1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC014271
Gene Symbol ENG
Gene ID (NCBI) 2022
RRIDAB_2881066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 28117-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 28117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Mol Cancer Res

A TGFβ-dependent stromal subset underlies immune checkpoint inhibitor efficacy in DNA mismatch repair-deficient/microsatellite instability-high colorectal cancer.

Authors - Eisei Endo
humanIHC

Sci Rep

Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells

Authors - Kazuma Yoshida