EIF4A2 Polyclonal antibody

EIF4A2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11280-1-AP

Print datasheet

Synonyms

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F



经过测试的应用

Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11280-1-AP targets EIF4A2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen EIF4A2 fusion protein Ag1810 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4A, isoform 2
Synonyms BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC015842
Gene Symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2097378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

实验方案

Product Specific Protocols
WB protocol for EIF4A2 antibody 11280-1-APDownload protocol
IHC protocol for EIF4A2 antibody 11280-1-APDownload protocol
IP protocol for EIF4A2 antibody 11280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC, WB

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
ratWB

Mol Ther Nucleic Acids

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.

Authors - Zhijie Shen
mouseWB

Stem Cell Rev Rep

Hyaluronan-CD44 Interaction Regulates Mouse Retinal Progenitor Cells Migration, Proliferation and Neuronal Differentiation

Authors - Jian Ma
humanWB

Mol Carcinog

miR-181a/b Induces the Growth, Invasion and Metastasis of Neuroblastoma Cells Through Targeting ABI1.

Authors - Xiaodan Liu
mouseWB,IF

Biol Reprod

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Authors - Jianjun Hu
humanWB

Microbes Infect

eIF4A2 is a host factor required for efficient HIV-1 replication.

Authors - Jerry Kwame Ndzinu