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  • KD/KO Validated

EIF3I Polyclonal antibody

EIF3I Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF, IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11287-1-AP

Print datasheet

Synonyms

eIF 3 beta, eIF3 beta, eIF3 p36, EIF3I, EIF3S2, PRO2242, TRIP 1, TRIP1



经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells, HeLa cells, MCF-7 cells, K-562 cells, human placenta tissue, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy EIF3I Ready-To-Use IHC Kit. EIF3I primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11287-1-AP targets EIF3I in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB,ELISA
Cited Applications CoIP, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Immunogen EIF3I fusion protein Ag1847 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit I
Synonyms eIF 3 beta, eIF3 beta, eIF3 p36, EIF3I, EIF3S2, PRO2242, TRIP 1, TRIP1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000413
Gene Symbol EIF3I
Gene ID (NCBI) 8668
RRIDAB_2097068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13347
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.

实验方案

Product Specific Protocols
WB protocol for EIF3I antibody 11287-1-APDownload protocol
IHC protocol for EIF3I antibody 11287-1-APDownload protocol
IF protocol for EIF3I antibody 11287-1-APDownload protocol
IP protocol for EIF3I antibody 11287-1-APDownload protocol
FC protocol for EIF3I antibody 11287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Metab Eng

Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis.

Authors - Anne Roobol
ratIHC,WB

J Bone Miner Res

TRIP-1: a regulator of osteoblast function.

Authors - Metz-Estrella Diana D
  • KD Validated
mouse, hamsterWB

Biochem J

The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.

Authors - Anne Roobol
humanWB,IF,CoIP

Ecotoxicol Environ Saf

Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.

Authors - Yujing Zhang
humanWB

Biochem J

Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.

Authors - Emma Jane Mead
humanWB

Vet Microbiol

EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein.

Authors - Wei Pan
  • KD Validated