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EIF3H Polyclonal antibody
EIF3H Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, PC-12 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11310-1-AP targets EIF3H in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | EIF3H fusion protein Ag1850 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 3, subunit H |
| Synonyms | eIF 3 gamma, eIF3 gamma, eIF3 p40, eIF3 p40 subunit, EIF3H, EIF3S3 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC000386 |
| Gene Symbol | EIF3H |
| Gene ID (NCBI) | 8667 |
| RRID | AB_2277723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15372 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3H is a component of the EIF3 complex. [PMID:19204574,20503360]
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF3H antibody 11310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.
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Biosci Biotechnol Biochem The proliferation of colorectal cancer cells is suppressed by silencing of EIF3H.
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Exp Ther Med Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis.
| ||
Nat Commun METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions | ||
Cell Commun Signal The deubiquitinase EIF3H promotes hepatocellular carcinoma progression by stabilizing OGT and inhibiting ferroptosis
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