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DST Polyclonal antibody

DST Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 28438-1-AP

Print datasheet

Synonyms

BP240, BPA, BPAG1, Bullous pemphigoid antigen, Bullous pemphigoid antigen 1, CATX 15, D6S1101, DKFZp564B2416, DMH, DST, DT, Dystonia musculorum protein, dystonin, FLJ46791, Hemidesmosomal plaque protein, KIAA0465, KIAA0728, KIAA1470, MACF2

验证数据展示

  • IHC staining of mouse brain using 28438-1-AP

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28438-1-AP

    IHC staining of mouse brain using 28438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 28438-1-AP

    IF Staining of SH-SY5Y using 28438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 28438-1-AP (DST antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 28438-1-AP

    WB analysis using 28438-1-AP

    Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28438-1-AP

    WB analysis using 28438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28438-1-AP targets DST in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Tested Reactivity Human, Mouse, rat
Immunogen DST fusion protein Ag28833 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dystonin
Synonyms BP240, BPA, BPAG1, Bullous pemphigoid antigen, Bullous pemphigoid antigen 1, CATX 15, D6S1101, DKFZp564B2416, DMH, DST, DT, Dystonia musculorum protein, dystonin, FLJ46791, Hemidesmosomal plaque protein, KIAA0465, KIAA0728, KIAA1470, MACF2
Calculated Molecular Weight 861 kDa
GenBank Accession NumberNM_001144769
Gene Symbol DST
Gene ID (NCBI) 667
RRIDAB_2881141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03001
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DST antibody 28438-1-APDownload protocol
IHC protocol for DST antibody 28438-1-APDownload protocol
IF protocol for DST antibody 28438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols