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DPYD Polyclonal antibody

DPYD Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 27662-1-AP

Print datasheet

Synonyms

DHP, DHPDHASE, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPD, DPYD

验证数据展示

  • WB analysis of rat liver using 27662-1-AP

    WB analysis of rat liver using 27662-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27662-1-AP

    IHC staining of human liver cancer using 27662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DPYD antibody included
  • IHC staining of human liver cancer using 27662-1-AP

    IHC staining of human liver cancer using 27662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DPYD antibody included

经过测试的应用

Positive WB detected inrat liver tissue, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DPYD Ready-To-Use IHC Kit. DPYD primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

27662-1-AP targets DPYD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB,ELISA
Cited Applications IHC
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen DPYD fusion protein Ag26599 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydropyrimidine dehydrogenase
Synonyms DHP, DHPDHASE, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPD, DPYD
Calculated Molecular Weight 1025 aa, 111 kDa
Observed Molecular Weight 111 kDa
GenBank Accession NumberBC008379
Gene Symbol DPYD
Gene ID (NCBI) 1806
RRIDAB_2880941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12882
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPYD antibody 27662-1-APDownload protocol
IHC protocol for DPYD antibody 27662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Pharmgenomics Pers Med

Exploration and Validation of Pancreatic Cancer Hub Genes Based on Weighted Gene Co-Expression Network Analysis and Immune Infiltration Score Analysis

Authors - Xiao-Xi Li
View Article