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DNAJB1 Monoclonal antibody

DNAJB1 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E8E10

Cat No : 67422-1-Ig

Print datasheet

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1

验证数据展示

  • WB analysis using 67422-1-Ig

    WB analysis using 67422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 67422-1-Ig

    FC experiment of HeLa using 67422-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67422-1-Ig targets DNAJB1 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, WB, ELISA
Cited Applications WB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen DNAJB1 fusion protein Ag3830 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Synonyms DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1
Calculated Molecular Weight 340 aa, 40 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC019827
Gene Symbol DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2882662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

实验方案

Product Specific Protocols
WB protocol for DNAJB1 antibody 67422-1-IgDownload protocol
FC protocol for DNAJB1 antibody 67422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

An Important Role for RPRD1B in the Heat Shock Response

Authors - Simona Cugusi
View Article