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DMBT1 Polyclonal antibody

DMBT1 Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 27069-1-AP

Print datasheet

Synonyms

DMBT1, Glycoprotein 340, Gp 340, GP340, Hensin, muclin, SAG, Salivary agglutinin, SALSA

验证数据展示

  • IHC staining of human stomach cancer using 27069-1-AP

    IHC staining of human stomach cancer using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 27069-1-AP

    IHC staining of human stomach cancer using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human brain using 27069-1-AP

    IF Staining of human brain using 27069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using DMBT1 antibody (27069-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human brain using 27069-1-AP

    IF Staining of human brain using 27069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using DMBT1 antibody (27069-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman stomach cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27069-1-AP is DMBT1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human
Immunogen DMBT1 fusion protein Ag16229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deleted in malignant brain tumors 1
Synonyms DMBT1, Glycoprotein 340, Gp 340, GP340, Hensin, muclin, SAG, Salivary agglutinin, SALSA
Calculated Molecular Weight 2413 aa, 261 kDa
GenBank Accession NumberBC153299
Gene Symbol DMBT1
Gene ID (NCBI) 1755
RRIDAB_2880741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Deleted in malignant brain tumors 1 (DMBT1), also known as the salivary scavenger and agglutinin (SALSA), salivary agglutinin (SAG) or gp340, is a multifunctional molecule with important functions in innate immunity, inflammation and epithelial homeostasis (PMID: 28668353). DMBT1 has some isoforms with the molecular weight of 193, 260 and 340 kDa.

实验方案

Product Specific Protocols
IHC protocol for DMBT1 antibody 27069-1-APDownload protocol
IF protocol for DMBT1 antibody 27069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols