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RIG-1/DDX58 Polyclonal antibody

RIG-1/DDX58 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25068-1-AP

Print datasheet

Synonyms

DDX58, DEAD box protein 58, RIG 1, RIG I

验证数据展示

  • WB analysis using 25068-1-AP

    WB analysis using 25068-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 25068-1-AP

    WB analysis of A431 using 25068-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25068-1-AP (DDX58 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 25068-1-AP

    WB analysis using 25068-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 25068-1-AP

    WB analysis using 25068-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 25068-1-AP

    IP experiment of A431 using 25068-1-AP

    IP result of anti-RIG-1/DDX58 (IP:25068-1-AP, 4ug; Detection:25068-1-AP 1:600) with A431 cells lysate 1200ug.

  • IHC staining of human heart using 25068-1-AP

    IHC staining of human heart using 25068-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 25068-1-AP

    IHC staining of human heart using 25068-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, THP-1 cells, NIH/3T3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25068-1-AP targets RIG-1/DDX58 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RIG-1/DDX58 fusion protein Ag18585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Synonyms DDX58, DEAD box protein 58, RIG 1, RIG I
Calculated Molecular Weight 925 aa, 107 kDa
Observed Molecular Weight 101/106 kDa
GenBank Accession NumberBC132786
Gene Symbol DDX58
Gene ID (NCBI) 23586
RRIDAB_2879881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95786
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

实验方案

Product Specific Protocols
WB protocol for RIG-1/DDX58 antibody 25068-1-APDownload protocol
IHC protocol for RIG-1/DDX58 antibody 25068-1-APDownload protocol
IP protocol for RIG-1/DDX58 antibody 25068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cancer Med

RIG-I promotes IFN/JAK2 expression and the endoplasmic reticulum stress response to inhibit chemoradiation resistance in nasopharyngeal carcinoma.

Authors - Di Jing
View Article