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- KD/KO Validated
RIG-1/DDX58 Polyclonal antibody
RIG-1/DDX58 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, A431 cells, MCF-7 cells, mouse brain tissue, HepG2 cells, MOLT-4 cells |
Positive IP detected in | HepG2 cells |
Positive IF detected in | HepG2 cells, H9C2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20566-1-AP targets RIG-1/DDX58 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IP, WB, ELISA |
Cited Applications | CoIP, IHC, IP, RIP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey, pig |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
Synonyms | DDX58, DEAD box protein 58, RIG 1, RIG I, RIG-I |
Calculated Molecular Weight | 107 kDa |
Observed Molecular Weight | 101/106 kDa |
GenBank Accession Number | NM_014314 |
Gene Symbol | DDX58 |
Gene ID (NCBI) | 23586 |
RRID | AB_10700006 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95786 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
IF protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
IP protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nat Microbiol IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. | ||
Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. | ||
EBioMedicine An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation | ||