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CoraLite® Plus 488-conjugated DACH1 Monoclonal antibody

DACH1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6D2

Cat No : CL488-60082

Print datasheet

Synonyms

DACH, DACH1, Dachshund homolog 1, FLJ10138

验证数据展示

  • IF Staining of mouse brain using CL488-60082

    IF Staining of mouse brain using CL488-60082

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

  • IF Staining of mouse brain using CL488-60082

    IF Staining of mouse brain using CL488-60082

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

经过测试的应用

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 60082-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60082 targets DACH1 in IF applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF/ICC
Tested Reactivity human, rat, mouse
Immunogen DACH1 fusion protein Ag4474 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dachshund homolog 1 (Drosophila)
Synonyms DACH, DACH1, Dachshund homolog 1, FLJ10138
Calculated Molecular Weight 79 kDa
GenBank Accession NumberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
RRIDAB_2883099
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UI36
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110 kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DACH1 antibody CL488-60082Download protocol
Standard Protocols
Click here to view our Standard Protocols