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Cytohesin 2 Monoclonal antibody

Cytohesin 2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A7A1

Cat No : 67185-1-Ig

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Synonyms

ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like



经过测试的应用

Positive WB detected inHepG2 cells, 4T1 cells, NIH/3T3 cells, PC-12 cells, HSC-T6 cells, A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67185-1-Ig targets Cytohesin 2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Cytohesin 2 fusion protein Ag21279 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cytohesin 2
Synonyms ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC004361
Gene Symbol CYTH2
Gene ID (NCBI) 9266
RRIDAB_2882481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.

实验方案

Product Specific Protocols
WB protocol for Cytohesin 2 antibody 67185-1-IgDownload protocol
IHC protocol for Cytohesin 2 antibody 67185-1-IgDownload protocol
IF protocol for Cytohesin 2 antibody 67185-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Elife

Localization of KRAS downstream target ARL4C to invasive pseudopods accelerates pancreatic cancer cell invasion.

Authors - Akikazu Harada
mouseWB

Pharmacol Res

Blocking the cytohesin-2/ARF1 axis by SecinH3 ameliorates osteoclast-induced bone loss via attenuating JNK-mediated IRE1 endoribonuclease activity

Authors - Yimin Dong
  • KD Validated