Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D7A9

Cat No : 66496-1-Ig

Print datasheet

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR



经过测试的应用

Positive WB detected inpig brain tissue, mouse brain tissue, rat brain tissue, U2OS cells, rat cerebellum tissue, U-251 cells, mouse cerebellum tissue, rabbit brain tissue
Positive IHC detected inhuman appendicitis tissue, human brain tissue, human cerebellum tissue, human colon tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, human appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66496-1-Ig targets Calretinin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity Human, mouse, pig, rat
Cited Reactivityhuman, mouse
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 2
Synonyms 29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol CALB2
Gene ID (NCBI) 794
RRIDAB_2868480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

实验方案

Product Specific Protocols
WB protocol for Calretinin antibody 66496-1-IgDownload protocol
IHC protocol for Calretinin antibody 66496-1-IgDownload protocol
IF protocol for Calretinin antibody 66496-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou
mouseWB,IF

Acta Neuropathol Commun

TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.

Authors - Tuancheng Feng
mouseWB,IF

Neurobiol Dis

Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury.

Authors - Charles R Lechner
mouseIF

Neurobiol Dis

Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord.

Authors - Akiko Ito
humanWB

J Cancer

A Prognostic Model of Angiogenesis and Neutrophil Extracellular Traps Related Genes Manipulating Tumor Microenvironment in Colon Cancer

Authors - Dongsheng Zhang
mouseIF

Invest Ophthalmol Vis Sci

Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Authors - Kei Akaiwa