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CX3CR1 Polyclonal antibody

CX3CR1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

FC, IF, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 13885-1-AP

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Synonyms

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, V28



经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, HEK-293 cells, SH-SY5Y cells, U-937 cells, K-562 cells
Positive IP detected inSH-SY5Y cells
Positive IF detected inmouse kidney tissue
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13885-1-AP targets CX3CR1 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF, IP, WB, ELISA
Cited ApplicationsCell treatment, CoIP, FC, IF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen CX3CR1 fusion protein Ag4149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-X3-C motif) receptor 1
Synonyms Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, V28
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 44-47 kDa
GenBank Accession NumberBC028078
Gene Symbol CX3CR1
Gene ID (NCBI) 1524
RRIDAB_2087418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

实验方案

Product Specific Protocols
WB protocol for CX3CR1 antibody 13885-1-APDownload protocol
IF protocol for CX3CR1 antibody 13885-1-APDownload protocol
IP protocol for CX3CR1 antibody 13885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP

Brain

Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.

Authors - Yun Xia
humanWB,IF

Tissue Cell

Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems

Authors - Mayuko Takata
mouseWB

J Control Release

Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme.

Authors - Sai Qiao
mouseWB

Nanoscale

Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles.

Authors - Liang Ding
humanWB

Mol Oncol

Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.

Authors - Shuo Miao
mouseWB,Cell treatment

J Nanobiotechnology

Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM.

Authors - Bao Liu