CTBP1 Monoclonal antibody
CTBP1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C4B2
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HEK-293 cells, A549 cells, HL-60 cells, HSC-T6 cells, HeLa cells, NCCIT cells, LNCaP cells, Jurkat cells, 4T1 cells, RAW 264.7 cells, NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:2000-1:8000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66995-1-Ig targets CTBP1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Tested Reactivity | Human, rat, mouse |
Immunogen | CTBP1 fusion protein Ag28567 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | C-terminal binding protein 1 |
Synonyms | BARS, C terminal binding protein 1, CTBP, CTBP1 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 45-48 kDa |
GenBank Accession Number | BC011655 |
Gene Symbol | CTBP1 |
Gene ID (NCBI) | 1487 |
RRID | AB_2882312 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13363 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CTBP1 antibody 66995-1-Ig | Download protocol |
IHC protocol for CTBP1 antibody 66995-1-Ig | Download protocol |
IF protocol for CTBP1 antibody 66995-1-Ig | Download protocol |
FC protocol for CTBP1 antibody 66995-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |