CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C4B2

Cat No : 66995-1-Ig

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Synonyms

BARS, C terminal binding protein 1, CTBP, CTBP1



经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, A549 cells, HL-60 cells, HSC-T6 cells, HeLa cells, NCCIT cells, LNCaP cells, Jurkat cells, 4T1 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66995-1-Ig targets CTBP1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC, IF, IHC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen CTBP1 fusion protein Ag28567 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name C-terminal binding protein 1
Synonyms BARS, C terminal binding protein 1, CTBP, CTBP1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC011655
Gene Symbol CTBP1
Gene ID (NCBI) 1487
RRIDAB_2882312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13363
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

实验方案

Product Specific Protocols
WB protocol for CTBP1 antibody 66995-1-IgDownload protocol
IHC protocol for CTBP1 antibody 66995-1-IgDownload protocol
IF protocol for CTBP1 antibody 66995-1-IgDownload protocol
FC protocol for CTBP1 antibody 66995-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols