CSTF1 Polyclonal antibody

CSTF1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10064-2-AP

Print datasheet

Synonyms

CF 1 50 kDa subunit, Cleavage stimulation factor, CstF 50, CSTF 50 kDa subunit, CSTF1, CstFp50



经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

10064-2-AP targets CSTF1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CSTF1 fusion protein Ag0109 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Synonyms CF 1 50 kDa subunit, Cleavage stimulation factor, CstF 50, CSTF 50 kDa subunit, CSTF1, CstFp50
Calculated Molecular Weight 48.3 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC001011
Gene Symbol CSTF1
Gene ID (NCBI) 1477
RRIDAB_2261145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05048
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.

实验方案

Product Specific Protocols
WB protocol for CSTF1 antibody 10064-2-APDownload protocol
IP protocol for CSTF1 antibody 10064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.

Authors - Patricia Uguen
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
humanWB

Oncogene

p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex.

Authors - F I Nazeer

Andrology

Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.

Authors - J Dai
rat,mouse,human

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min