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CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10225-1-AP

Print datasheet

Synonyms

CRABP II, CRABP2, RBP6



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, human skin cancer tissue
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10225-1-AP targets CRABP2 in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CRABP2 fusion protein Ag0309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cellular retinoic acid binding protein 2
Synonyms CRABP II, CRABP2, RBP6
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC001109
Gene Symbol CRABP2
Gene ID (NCBI) 1382
RRIDAB_2085455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29373
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

实验方案

Product Specific Protocols
WB protocol for CRABP2 antibody 10225-1-APDownload protocol
IHC protocol for CRABP2 antibody 10225-1-APDownload protocol
IF protocol for CRABP2 antibody 10225-1-APDownload protocol
IP protocol for CRABP2 antibody 10225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

A single progenitor population switches behavior to maintain and repair esophageal epithelium.

Authors - David P Doupé
humanWB

Mol Cell

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Authors - Ai Kaiho-Soma
humanWB,IF

Neuron

Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.

Authors - Alana I Mendelsohn
mouseIF

Nat Commun

The developing mouse coronal suture at single-cell resolution.

Authors - D'Juan T Farmer
mouseIF

Dev Cell

Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.

Authors - John DeSisto
humanIF

Stem Cell Reports

BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions.

Authors - Kai Kretzschmar