CPEB3 Polyclonal antibody

CPEB3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12669-1-AP

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Synonyms

CPE binding protein 3, CPE BP3, CPEB3, hCPEB 3, KIAA0940



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy CPEB3 Ready-To-Use IHC Kit. CPEB3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12669-1-AP targets CPEB3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CPEB3 fusion protein Ag3351 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytoplasmic polyadenylation element binding protein 3
Synonyms CPE binding protein 3, CPE BP3, CPEB3, hCPEB 3, KIAA0940
Calculated Molecular Weight 697 aa, 76 kDa
Observed Molecular Weight 76-80 kDa
GenBank Accession NumberBC036444
Gene Symbol CPEB3
Gene ID (NCBI) 22849
RRIDAB_2877871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NE35
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPEB3, the cytoplasmic polyadenylation element-binding protein 3, contains a Q/N-rich domain at its N-termina which is required for the aggregation of CPEB3. The expression of CPEB3 is downregulated in cervical cancer and hepatocellular carcinoma compared with normal tissue. It has been reported that the aggregation and activity of CPEB3 is controlled by SUMOylation. CPEB3 is SUMOylated in hippocampal neurons in the basal state while converted into an active form which is associated with a decrease of SUMOylation and an increase of aggregation. CPEB3 is expressed in cytoplasma in unstimulated neurons but translocates to the nucleus following neuronal stimulation.

实验方案

Product Specific Protocols
WB protocol for CPEB3 antibody 12669-1-APDownload protocol
IHC protocol for CPEB3 antibody 12669-1-APDownload protocol
IF protocol for CPEB3 antibody 12669-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pharmacology

MiR-224 Executes a Tumor Accelerative Role during Hepatocellular Carcinoma Malignancy by Targeting Cytoplasmic Polyadenylation Element-Binding Protein 3.

Authors - Ke Miao