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COXIV Polyclonal antibody

COXIV Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11242-1-AP

Print datasheet

Synonyms

Cell and organelle markers, COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Mitochondrion Marker



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, rat liver tissue, rat brain tissue, MCF-7 cells, NIH/3T3 cells, Jurkat cells, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman prostate cancer tissue, human pancreas cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11242-1-AP targets COXIV in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications ChIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, pig, canine
Immunogen COXIV fusion protein Ag1640 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome c oxidase subunit IV isoform 1
Synonyms Cell and organelle markers, COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Mitochondrion Marker
Calculated Molecular Weight19.6 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC021236
Gene Symbol COX4I1
Gene ID (NCBI) 1327
RRIDAB_2085278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13073
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.

实验方案

Product Specific Protocols
WB protocol for COXIV antibody 11242-1-APDownload protocol
IHC protocol for COXIV antibody 11242-1-APDownload protocol
IF protocol for COXIV antibody 11242-1-APDownload protocol
IP protocol for COXIV antibody 11242-1-APDownload protocol
FC protocol for COXIV antibody 11242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
human,mouseWB,IF

Immunity

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Authors - Fangzhou Lou
humanWB

Cell Metab

Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.

Authors - Kiran Kurmi
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
mouseWB

Cell Res

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Authors - Tingting Hou
humanIF

Nucleic Acids Res

Restoration of mitochondrial function through activation of hypomodified tRNAs with pathogenic mutations associated with mitochondrial diseases

Authors - Ena Tomoda