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COPS7A Polyclonal antibody

COPS7A Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16628-1-AP

Print datasheet

Synonyms

COPS7A, CSN7A, DERP10, SGN7a, Signalosome subunit 7a

验证数据展示

  • WB analysis using 16628-1-AP

    WB analysis using 16628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16628-1-AP

    IP experiment of HeLa using 16628-1-AP

    IP result of anti-COPS7A (IP:16628-1-AP, 4ug; Detection:16628-1-AP 1:5000) with HeLa cells lysate 1600 ug.

  • IHC staining of mouse brain using 16628-1-AP

    IHC staining of mouse brain using 16628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16628-1-AP (COPS7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 16628-1-AP

    IF Staining of MCF-7 using 16628-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using COPS7A antibody (16628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse brain tissue, PC-12 cells, HeLa cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16628-1-AP targets COPS7A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen COPS7A fusion protein Ag9958 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis)
Synonyms COPS7A, CSN7A, DERP10, SGN7a, Signalosome subunit 7a
Calculated Molecular Weight 275 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC011789
Gene Symbol COPS7A
Gene ID (NCBI) 50813
RRIDAB_2081787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPS7A is component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. COPS7A was consistently expressed in oligodendrocyte lineage cells, and was down-regulated during the peak period of myelination. COPS7A associated physically with Olig1, and it might thus serve as a novel negative regulator in oligodendrocytes differentiation.

实验方案

Product Specific Protocols
WB protocol for COPS7A antibody 16628-1-APDownload protocol
IHC protocol for COPS7A antibody 16628-1-APDownload protocol
IF protocol for COPS7A antibody 16628-1-APDownload protocol
IP protocol for COPS7A antibody 16628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols