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CERK Polyclonal antibody

CERK Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25731-1-AP

Print datasheet

Synonyms

Acylsphingosine kinase, ceramide kinase, CERK, dA59H18.2, dA59H18.3, hCERK, KIAA1646, Lipid kinase 4, LK4

验证数据展示

  • WB analysis of mouse heart using 25731-1-AP

    WB analysis of mouse heart using 25731-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 25731-1-AP

    IHC staining of mouse heart using 25731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 25731-1-AP

    IHC staining of mouse heart using 25731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IHC detected inmouse heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25731-1-AP is CERK Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen CERK fusion protein Ag22774 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ceramide kinase
Synonyms Acylsphingosine kinase, ceramide kinase, CERK, dA59H18.2, dA59H18.3, hCERK, KIAA1646, Lipid kinase 4, LK4
Calculated Molecular Weight 537 aa, 60 kDa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC126940
Gene Symbol CERK
Gene ID (NCBI) 64781
RRIDAB_2880214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCT0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.

实验方案

Product Specific Protocols
WB protocol for CERK antibody 25731-1-APDownload protocol
IHC protocol for CERK antibody 25731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols