CDC25C Monoclonal antibody
CDC25C Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (2)
Applications
FC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2B6B12
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Raji cells |
Positive FC detected in | K-562 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
产品信息
66912-1-Ig targets CDC25C in WB, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | FC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse |
Immunogen | CDC25C fusion protein Ag10240 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | cell division cycle 25 homolog C (S. pombe) |
Synonyms | CDC25, CDC25C, M phase inducer phosphatase 3 |
Calculated Molecular Weight | 473 aa, 53 kDa |
Observed Molecular Weight | 46-53 kDa |
GenBank Accession Number | BC019089 |
Gene Symbol | CDC25C |
Gene ID (NCBI) | 995 |
RRID | AB_2882239 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P30307 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CDC25C antibody 66912-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Reprod Toxicol High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation. | ||
Oncol Lett Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G2/M cell cycle arrest and apoptosis. | ||
Food Chem Toxicol DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells | ||
Toxicology ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice |