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CD79B Polyclonal antibody

CD79B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21063-1-AP

Print datasheet

Synonyms

B29, CD79B, Ig beta, IGB

验证数据展示

  • WB analysis of Raji using 21063-1-AP

    WB analysis of Raji using 21063-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21063-1-AP (CD79B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21063-1-AP

    IHC staining of human tonsillitis using 21063-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 21063-1-AP

    IHC staining of human tonsillitis using 21063-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

The immunogen of 21063-1-AP is CD79B Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited Applications IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CD79B fusion protein Ag15086 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD79b molecule, immunoglobulin-associated beta
Synonyms B29, CD79B, Ig beta, IGB
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC032651
Gene Symbol CD79B
Gene ID (NCBI) 974
RRIDAB_2878803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CD79B antibody 21063-1-APDownload protocol
IHC protocol for CD79B antibody 21063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Immunol

CD20+CD22+ADAM28+ B Cells in Tertiary Lymphoid Structures Promote Immunotherapy Response.

Authors - Zhenghao Wu
View Article
mouseIF

Cell Discov

Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure

Authors - Jie Yin
View Article