CD5 Monoclonal antibody

CD5 Monoclonal Antibody for IF, IHC,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4D12C10

Cat No : 67278-1-Ig

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Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1



经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67278-1-Ig targets CD5 in IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC,ELISA
Tested Reactivity Human
Immunogen CD5 fusion protein Ag11065 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD5 molecule
Synonyms CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1
Calculated Molecular Weight 495 aa, 55 kDa
GenBank Accession NumberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
RRIDAB_2882546
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06127
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
IHC protocol for CD5 antibody 67278-1-IgDownload protocol
IF protocol for CD5 antibody 67278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols