CoraLite® Plus 488-conjugated CD31 Monoclonal antibody
CD31 Monoclonal Antibody for FC, IF
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
FC, IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3F8E2
验证数据展示
经过测试的应用
Positive IF detected in | Lymphatic endothelial cells cells, human colon cancer tissue, human tonsillitis tissue |
Positive FC detected in | THP-1 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 2 publications below |
产品信息
CL488-66065 targets CD31 in IF, FC applications and shows reactivity with human samples.
Tested Applications | FC, IF |
Cited Applications | IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | CD31 fusion protein Ag1787 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | platelet/endothelial cell adhesion molecule |
Synonyms | CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker |
Calculated Molecular Weight | 83 kDa |
GenBank Accession Number | BC022512 |
Gene Symbol | PECAM1 |
Gene ID (NCBI) | 5175 |
ENSEMBL Gene ID | ENSG00000261371 |
RRID | AB_2883247 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P16284 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 CD31 antibody CL488-66065 | Download protocol |
FC protocol for CL Plus 488 CD31 antibody CL488-66065 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biochem Biophys Res Commun Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage | ||
Cell Death Dis Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1 |