CoraLite®488-conjugated CD13 Monoclonal antibody

CD13 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra), IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2D8D11

Cat No : CL488-66211

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Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN



经过测试的应用

Positive IF detected inhuman kidney tissue
Positive FC detected inU-937 cells
For other applications, we recommend the unconjugated version of this antibody, 66211-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66211 targets CD13 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF
Tested Reactivity human
Immunogen CD13 fusion protein Ag5976 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name alanyl (membrane) aminopeptidase
Synonyms Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC058928
Gene Symbol ANPEP
Gene ID (NCBI) 290
ENSEMBL Gene IDENSG00000166825
RRIDAB_2883285
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15144
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174) And this antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

实验方案

Product Specific Protocols
IF protocol for CL488 CD13 antibody CL488-66211Download protocol
FC protocol for CL488 CD13 antibody CL488-66211Download protocol
Standard Protocols
Click here to view our Standard Protocols