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CoraLite® Plus 647 Anti-Mouse CD117 (c-Kit) (ACK2)

CD117 (c-Kit) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

ACK2

Cat No : CL647-65094

Print datasheet

Synonyms

Bs, c KIT, CD117, CD117 (c-Kit), Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit

验证数据展示

  • FC experiment of mouse bone marrow cells using CL647-65094

    FC experiment of mouse bone marrow cells using CL647-65094

    1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD11b (FITC-65055, Clone: M1/70) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD117 (c-Kit) (CL647-65094, Clone: ACK2). Cells were not fixed.

  • FC experiment of mouse bone marrow cells using CL647-65094

    FC experiment of mouse bone marrow cells using CL647-65094

    1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD11b (FITC-65055, Clone: M1/70) and CoraLite® Plus 647-conjugated Rat IgG2b isotype control antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65094-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65094 targets CD117 (c-Kit) in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen Murine IL-3 dependent mast cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name kit oncogene
Synonyms Bs, c KIT, CD117, CD117 (c-Kit), Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit
GenBank Accession NumberBC052457
Gene Symbol Kit
Gene ID (NCBI) 16590
RRIDAB_2883678
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP05532
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD117 (c-Kit) antibody CL647-65094Download protocol
Standard Protocols
Click here to view our Standard Protocols