CoraLite® Plus 488 Anti-Mouse CD107a / LAMP1 (1D4B)

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

FC (Intra), IF and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D4B

Cat No : CL488-65050

Print datasheet

Synonyms

AI196048, CD107a, Lamp 1, Lamp1



经过测试的应用

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 65050-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

CL488-65050 targets CD107a / LAMP1 in IHC, IF, FC (Intra) applications and shows reactivity with Mouse samples.

Tested Applications FC (Intra), IF
Cited ApplicationsIHC
Tested Reactivity Mouse
Cited Reactivitymouse
Immunogen Plasma membrane fraction of mouse embryo NIH/3T3 cell line 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms AI196048, CD107a, Lamp 1, Lamp1
GenBank Accession NumberBC006785
Gene Symbol Lamp1
Gene ID (NCBI) 16783
RRIDAB_2883148
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP11438
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-65050Download protocol
FC protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-65050Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Research (Wash D C)

Unresolved Excess Accumulation of Myelin-Derived Cholesterol Contributes to Scar Formation after Spinal Cord Injury

Authors - Bolin Zheng