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Calpastatin Monoclonal antibody

Calpastatin Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G12A2

Cat No : 67107-1-Ig

Print datasheet

Synonyms

BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component

验证数据展示

  • WB analysis of HeLa using 67107-1-Ig

    WB analysis of HeLa using 67107-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (CAST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67107-1-Ig

    IF Staining of HeLa using 67107-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (67107-1-Ig, Clone: 1G12A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 67107-1-Ig

    FC experiment of HeLa using 67107-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calpastatin (67107-1-Ig, Clone:1G12A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, MCF-7 cells, THP-1 cells, HEK-293 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67107-1-Ig targets Calpastatin in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivitymouse
Immunogen Calpastatin fusion protein Ag28669 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpastatin
Synonyms BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component
Calculated Molecular Weight 667 aa, 72 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC013579
Gene Symbol CAST
Gene ID (NCBI) 831
RRIDAB_2882411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Calpastatin antibody 67107-1-IgDownload protocol
IF protocol for Calpastatin antibody 67107-1-IgDownload protocol
FC protocol for Calpastatin antibody 67107-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.

Authors - Fengming Shen
View Article
mouseWB

Pharmacol Res

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

Authors - Juan Wang
View Article