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  • KD/KO Validated

CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15112-1-AP

Print datasheet

Synonyms

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105



经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections
Positive FC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CAPRIN1 Ready-To-Use IHC Kit. CAPRIN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15112-1-AP targets CAPRIN1 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Immunogen CAPRIN1 fusion protein Ag7099 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell cycle associated protein 1
Synonyms caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC001731
Gene Symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2070016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

实验方案

Product Specific Protocols
WB protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IHC protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IF protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IP protocol for CAPRIN1 antibody 15112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
  • KD Validated
humanWB,IF

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
humanWB,IF

Nat Immunol

Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA.

Authors - Henan Xu
mouseWB,RIP

Nat Commun

Fear extinction is regulated by the activity of long noncoding RNAs at the synapse

Authors - Wei-Siang Liau
WB, IP

Neuron

CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.

Authors - Silvia De Rubeis