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Calpain 6 Polyclonal antibody

Calpain 6 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10120-1-AP

Print datasheet

Synonyms

calpain 6, Calpain like protease X linked, Calpamodulin, CalpM, CANPX, CAPN6, CAPNX

验证数据展示

  • WB analysis using 10120-1-AP

    WB analysis using 10120-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 10120-1-AP

    IP experiment of human placenta using 10120-1-AP

    IP result of anti-Calpain 6 (IP:10120-1-AP, 4ug; Detection:10120-1-AP 1:300) with human placenta tissue lysate 1520ug.

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse placenta tissue, A549 cells
Positive IP detected inhuman placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10120-1-AP targets Calpain 6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, rat
Immunogen Calpain 6 fusion protein Ag0160 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calpain 6
Synonyms calpain 6, Calpain like protease X linked, Calpamodulin, CalpM, CANPX, CAPN6, CAPNX
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 66-75 kDa
GenBank Accession NumberBC000730
Gene Symbol CAPN6
Gene ID (NCBI) 827
RRIDAB_2290829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Q1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Calpain 6 antibody 10120-1-APDownload protocol
IP protocol for Calpain 6 antibody 10120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

J Neuropathol Exp Neurol

MicroRNA Alteration in Developing Rat Oligodendrocyte Precursor Cells Induced by Hypoxia-Ischemia.

Authors - Xiaojuan Su
View Article
humanWB,IHC

Am J Reprod Immunol

The investigation of calpain in human placenta with fetal growth restriction.

Authors - Shinobu Goto
View Article
humanWB

Amino Acids

Advanced glycation end products promote the progression of chronic kidney diseases by targeting calpain 6

Authors - Yufan Zhang
View Article