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CAPG-Specific Polyclonal antibody

CAPG-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19535-1-AP

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Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP



经过测试的应用

Positive WB detected inmouse lung tissue, mouse kidney tissue, U-937 cells
Positive IHC detected inhuman spleen tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19535-1-AP targets CAPG-Specific in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name capping protein (actin filament), gelsolin-like
Synonyms Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberNM_001747
Gene Symbol CAPG
Gene ID (NCBI) 822
RRIDAB_10638618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

实验方案

Product Specific Protocols
WB protocol for CAPG-Specific antibody 19535-1-APDownload protocol
IHC protocol for CAPG-Specific antibody 19535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Tohoku J Exp Med

Overexpression of gelsolin-like actin-capping protein is associated with progression of lung adenocarcinoma.

Authors - Shao Fangchun F
  • KD Validated