Bcl-XL Polyclonal antibody

Bcl-XL Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

FC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26967-1-AP

Print datasheet

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-xL



经过测试的应用

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26967-1-AP targets Bcl-XL in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine
Immunogen Bcl-XL fusion protein Ag25604 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-like 1
Synonyms Apoptosis regulator Bcl X, BCL2L1, Bcl-xL
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC019307
Gene Symbol BCL2L1
Gene ID (NCBI) 598
RRIDAB_2880702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

实验方案

Product Specific Protocols
WB protocol for Bcl-XL antibody 26967-1-APDownload protocol
IHC protocol for Bcl-XL antibody 26967-1-APDownload protocol
IP protocol for Bcl-XL antibody 26967-1-APDownload protocol
FC protocol for Bcl-XL antibody 26967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Microbiome

The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.

Authors - Weiping Xiao
humanWB

Clin Cancer Res

Long noncoding RNA LINC01234 functions as a competing endogenous RNA to regulate CBFB expression by sponging miR-204-5p in gastric cancer.

Authors - Xin Chen
humanWB

Clin Cancer Res

Inhibition of Bcl-2 Synergistically Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Preclinical Models of FLT3-mutated Acute Myeloid Leukemia.

Authors - Jun Ma
humanWB

Cell Rep

Developmental potency of human ES cell-derived mesenchymal stem cells revealed in mouse embryos following blastocyst injection

Authors - Borong Huang
mouseWB

Int J Biol Macromol

CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression

Authors - Zhongqiu Zhou
humanWB,IHC

Cancer Lett

Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.

Authors - Ruqi Jiang