BDNF Polyclonal antibody

BDNF Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28205-1-AP

Print datasheet

Synonyms

Abrineurin, BDNF



经过测试的应用

Positive WB detected inC6 cells, SH-SY5Y cells, PC-12 cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28205-1-AP targets BDNF in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen BDNF fusion protein Ag28276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name brain-derived neurotrophic factor
Synonyms Abrineurin, BDNF
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
RRIDAB_2818984
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23560
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).


实验方案

Product Specific Protocols
WB protocol for BDNF antibody 28205-1-APDownload protocol
IHC protocol for BDNF antibody 28205-1-APDownload protocol
IF protocol for BDNF antibody 28205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

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Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration.

Authors - Paola Rusmini
mouseWB

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ACS Appl Mater Interfaces

Reactive Oxygen Species Scavenging Functional Hydrogel Delivers Procyanidins for the Treatment of Traumatic Brain Injury in Mice.

Authors - Xuyang Huang
mouseWB

Aging Dis

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

Authors - Minghao Yuan
ratWB

Neurobiol Stress

Low-dose S-ketamine exerts antidepressant-like effects via enhanced hippocampal synaptic plasticity in postpartum depression rats.

Authors - Zhuoyu Ren
mouseWB

Brain Behav Immun

IL-33 in the basolateral amygdala integrates neuroinflammation into anxiogenic circuits via modulating BDNF expression.

Authors - Xiao Zhuang