BCL9 Polyclonal antibody

BCL9 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22947-1-AP

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Synonyms

B cell CLL/lymphoma 9, B cell CLL/lymphoma 9 protein, B cell lymphoma 9 protein, BCL9, LGS, Protein legless homolog



经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22947-1-AP targets BCL9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen BCL9 fusion protein Ag19158 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 9
Synonyms B cell CLL/lymphoma 9, B cell CLL/lymphoma 9 protein, B cell lymphoma 9 protein, BCL9, LGS, Protein legless homolog
Calculated Molecular Weight 1426 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC116451
Gene Symbol BCL9
Gene ID (NCBI) 607
RRIDAB_2879189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

实验方案

Product Specific Protocols
WB protocol for BCL9 antibody 22947-1-APDownload protocol
IHC protocol for BCL9 antibody 22947-1-APDownload protocol
IF protocol for BCL9 antibody 22947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bioorg Chem

Discovery of 2-(isoxazol-5-yl)phenyl 3,4-dihydroxybenzoate as a potential inhibitor for the Wnt/β-catenin pathway

Authors - Yongxi Dong
human,mouseWB,IHC

Reprod Biol Endocrinol

Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.

Authors - Xi Wang
humanWB,IF,IP

J Biol Chem

USP9X-mediated deubiquitination of B cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.

Authors - Zesen Shang