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BAX Monoclonal antibody

BAX Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat and More (2)

Applications

FC, IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

4G5E8

Cat No : 60267-1-Ig

Print datasheet

Synonyms

Apoptosis regulator BAX, BAX



经过测试的应用

Positive WB detected inHeLa cells, human testis tissue, HepG2 cells, HEK-293 cells, COLO 320 cells, PC-12 cells, ROS1728 cells, Neuro-2a cells
Positive IP detected inTHP-1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy BAX Ready-To-Use IHC Kit. BAX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60267-1-Ig targets BAX in WB, IP, IHC, IF, ICC, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, IP, WB, ELISA
Cited Applications CoIP, ICC, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Immunogen BAX fusion protein Ag21068 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BCL2-associated X protein
Synonyms Apoptosis regulator BAX, BAX
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC014175
Gene Symbol BAX
Gene ID (NCBI) 581
RRIDAB_2848213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07812
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).

实验方案

Product Specific Protocols
WB protocol for BAX antibody 60267-1-IgDownload protocol
IHC protocol for BAX antibody 60267-1-IgDownload protocol
IP protocol for BAX antibody 60267-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,ICC

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
ratWB

J Adv Res

Evolocumab prevents atrial fibrillation in rheumatoid arthritis rats through restraint of PCSK9 induced atrial remodeling

Authors - Xuejie Han
humanWB

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
mouseWB,IF

Cell Death Dis

DUSP1 protects against ischemic acute kidney injury through stabilizing mtDNA via interaction with JNK

Authors - Lang Shi
mouseWB

Theranostics

Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.

Authors - Lujing Jiang
mouseWB

Cell Prolif

OGG1 aggravates renal ischemia-reperfusion injury by repressing PINK1-mediated mitophagy

Authors - Fan Zhao