B3GNT6 Polyclonal antibody

B3GNT6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21291-1-AP

Print datasheet

Synonyms

B3Gn T6, B3GNT6, Beta 1,3 Gn T6, Beta3Gn T6, BGnT 6, Core 3 synthase, IMAGE:4907098



经过测试的应用

Positive WB detected inmouse colon tissue, mouse small intestine tissue, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21291-1-AP is B3GNT6 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen B3GNT6 fusion protein Ag15825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
Synonyms B3Gn T6, B3GNT6, Beta 1,3 Gn T6, Beta3Gn T6, BGnT 6, Core 3 synthase, IMAGE:4907098
Calculated Molecular Weight 384 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC103910
Gene Symbol B3GNT6
Gene ID (NCBI) 192134
RRIDAB_2878834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZMB0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The B3GNT6 protein, also known as core 3 synthase, is a member of the O-GlcNAc transferase (OGT) family and adds an N-acetylglucosamine to N-acetylgalactosamine-modifed serine or threonine. The B3GNT6 protein is responsible for the formation of the core 3 structure of O-glycans, which are important components of mucin-type glycoproteins. This process is known as the protein O-GlcNAcylation modifcation. B3GNT6 expression has been reported to be largely downregulated in gastric and colorectal cancers. B3GNT6 has 3 isoforms with the molecular mass of 29 and 43 kDa. (PMID: 35387659)

实验方案

Product Specific Protocols
WB protocol for B3GNT6 antibody 21291-1-APDownload protocol
IHC protocol for B3GNT6 antibody 21291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Oncogene

Core 3 mucin-type O-glycan restoration in colorectal cancer cells promotes MUC1/p53/miR-200c-dependent epithelial identity.

Authors - J Ye
WB

Mol Ther Methods Clin Dev

An etanercept O-glycovariant with enhanced potency.

Authors - Thomas G Biel
human,mouseWB,IHC

Cancer Med

G-quadruplex-enhanced circular single-stranded DNA (G4-CSSD) adsorption of miRNA to inhibit colon cancer progression

Authors - Haidong Wu
ratWB

Int J Food Sci Nutr

Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats

Authors - Bo Peng