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CoraLite®594-conjugated Albumin Monoclonal antibody

Albumin Monoclonal Antibody for FC (Intra), IF/ICC,IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra), IF/ICC,IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4A1C11

Cat No : CL594-66051

Print datasheet

Synonyms

ALB, albumin, DKFZp779N1935, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341

验证数据展示

  • IF Staining of mouse liver using CL594-66051

    IF Staining of mouse liver using CL594-66051

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 Albumin antibody (CL594-66051, Clone: 4A1C11 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66051

    FC experiment of HepG2 using CL594-66051

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Albumin (CL594-66051, Clone:4A1C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • IF Staining of HepG2 using CL594-66051

    IF Staining of HepG2 using CL594-66051

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Albumin antibody (CL594-66051, Clone: 4A1C11 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66051

    FC experiment of HepG2 using CL594-66051

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Albumin (CL594-66051, Clone:4A1C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse liver tissue, HepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66051-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66051 targets Albumin in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF/ICC,IF-P
Tested Reactivity human, mouse
Immunogen Albumin fusion protein Ag9885 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name albumin
Synonyms ALB, albumin, DKFZp779N1935, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341
Calculated Molecular Weight 609 aa, 69 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC034023
Gene Symbol ALB
Gene ID (NCBI) 213
RRIDAB_2883493
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02768
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

实验方案

Product Specific Protocols
IF protocol for CL594 Albumin antibody CL594-66051Download protocol
Standard Protocols
Click here to view our Standard Protocols