- Featured Product
- KD/KO Validated
ATP13A1 Polyclonal antibody
ATP13A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
产品信息
16244-1-AP targets ATP13A1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF, IHC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ATP13A1 fusion protein Ag9174 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATPase type 13A1 |
| Synonyms | ATP13A, ATP13A1, ATPase type 13A1, CGI 152, KIAA1825 |
| Calculated Molecular Weight | 976 aa, 109 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC009302 |
| Gene Symbol | ATP13A1 |
| Gene ID (NCBI) | 57130 |
| RRID | AB_2290293 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| IHC protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| IF protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| IP protocol for ATP13A1 antibody 16244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.
| ||
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology | ||
J Cell Sci Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex.
| ||
J Biol Chem The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation. | ||
Cell Rep CATP-8/P5A ATPase Regulates ER Processing of the DMA-1 Receptor for Dendritic Branching. | ||