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- KD/KO Validated
ASPM Polyclonal antibody
ASPM Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF/ICC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, K-562 cells |
| Positive IHC detected in | human prostate cancer tissue, human gliomas tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 26223-1-AP is ASPM Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | CoIP, IF, IHC, IP, WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | ASPM fusion protein Ag24166 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | asp (abnormal spindle) homolog, microcephaly associated (Drosophila) |
| Synonyms | ASP, Asp homolog, ASPM, Calmbp1, MCPH5 |
| Calculated Molecular Weight | 410 kDa or 218 kDa |
| Observed Molecular Weight | 218 kDa |
| GenBank Accession Number | BC034607 |
| Gene Symbol | ASPM |
| Gene ID (NCBI) | 259266 |
| RRID | AB_2880431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASPM antibody 26223-1-AP | Download protocol |
| IHC protocol for ASPM antibody 26223-1-AP | Download protocol |
| IF protocol for ASPM antibody 26223-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) ASPM promotes glioblastoma growth by regulating G1 restriction point progression and Wnt-β-catenin signaling.
| ||
Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. | ||
Front Oncol Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis. | ||
J Biol Chem The Transcription factor NF-YA is Crucial for Neural Progenitor Maintenance during Brain Development | ||
DNA Cell Biol ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth. | ||
Int J Endocrinol ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway.
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