• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ART1 Monoclonal antibody

ART1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D10A8

Cat No : 66958-1-Ig

Print datasheet

Synonyms

ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6

验证数据展示

  • WB analysis using 66958-1-Ig

    WB analysis using 66958-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66958-1-Ig (ART1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inEC109 cells, HeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66958-1-Ig targets ART1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivitymouse
Immunogen ART1 fusion protein Ag28269 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADP-ribosyltransferase 1
Synonyms ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberNM_004314
Gene Symbol ART1
Gene ID (NCBI) 417
RRIDAB_2882281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52961
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.

实验方案

Product Specific Protocols
WB protocol for ART1 antibody 66958-1-IgDownload protocol
IHC protocol for ART1 antibody 66958-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Med Oncol

Identification of the role of mono-ADP-ribosylation in colorectal cancer by integrated transcriptome analysis.

Authors - Shuxian Zhang
View Article