CoraLite® Plus 488-conjugated Arginase-1 Monoclonal antibody
Arginase-1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5D6D12
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66129-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
The immunogen of CL488-66129 is Arginase-1 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC |
| Cited Applications | IF |
| Tested Reactivity | human, pig, rat, mouse |
| Cited Reactivity | mouse |
| Immunogen | Arginase-1 fusion protein Ag8810 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | arginase, liver |
| Synonyms | ARG1, Arginase 1, arginase, liver, Arginase-1, Liver type arginase, Type I arginase |
| Calculated Molecular Weight | 236aa,25 kDa; 322aa,35 kDa |
| GenBank Accession Number | BC005321 |
| Gene Symbol | ARG1 |
| Gene ID (NCBI) | 383 |
| RRID | AB_2883268 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P05089 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 is a novel biomarker of post-stroke immune suppression(2016). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Arginase-1 antibody CL488-66129 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Cancer Res 18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition. | ||
Biosci Rep Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis. |