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Arginase-1 Monoclonal antibody
Arginase-1 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5D6D12
验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, RAW 264.7 cells, mouse liver tissue, pig liver tissue |
| Positive IP detected in | rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66129-1-Ig targets Arginase-1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, pig, rat, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, pig, rat, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Arginase-1 fusion protein Ag8810 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | arginase, liver |
| Synonyms | ARG1, Arginase 1, arginase, liver, Arginase-1, liver Arginase, Liver type arginase |
| Calculated Molecular Weight | 236aa,25 kDa; 322aa,35 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC005321 |
| Gene Symbol | ARG1 |
| Gene ID (NCBI) | 383 |
| RRID | AB_2881528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05089 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Arginase-1 antibody 66129-1-Ig | Download protocol |
| IHC protocol for Arginase-1 antibody 66129-1-Ig | Download protocol |
| IF protocol for Arginase-1 antibody 66129-1-Ig | Download protocol |
| IP protocol for Arginase-1 antibody 66129-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. | ||
J Neuroinflammation Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization | ||
Int J Biol Macromol A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects | ||
Carbohydr Polym Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism | ||
Int J Nanomedicine Rosuvastatin Nanomicelles Target Neuroinflammation and Improve Neurological Deficit in a Mouse Model of Intracerebral Hemorrhage. | ||
NPJ Parkinsons Dis Melatonin ameliorates Parkinson's disease via regulating microglia polarization in a RORα-dependent pathway. |
